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placzi  (TaKaRa)


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    TaKaRa placzi
    Placzi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placzi+vector/10__1016_slash_j__cj__2025__06__002-81-26-28?v=TaKaRa
    Average 94 stars, based on 15 article reviews
    placzi - by Bioz Stars, 2026-07
    94/100 stars

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    (A) SPL9 interacts with <t>the</t> <t>MAC3A</t> and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the <t>pLacZi</t> promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
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    (A) SPL9 interacts with <t>the</t> <t>MAC3A</t> and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the <t>pLacZi</t> promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
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    (A) SPL9 interacts with the MAC3A and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the pLacZi promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.

    Journal: bioRxiv

    Article Title: MOS4-Associated Complex subunits 3A and 3B modulate FLM splicing to repress photoperiod-dependent floral transition

    doi: 10.1101/2024.03.26.586198

    Figure Lengend Snippet: (A) SPL9 interacts with the MAC3A and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the pLacZi promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.

    Article Snippet: The promoter regions of MAC3A (−1487 to +266) and MAC3B (−1487 to +266) were cloned into pLacZi-GW reporter vectors, which incorporate a Gateway cassette between the SmaI and XhoI sites of the original pLacZi vectors (Clontech).

    Techniques: Expressing, Activity Assay, Negative Control, Chromatin Immunoprecipitation, Binding Assay, Labeling, Immunoprecipitation